Coding
Cah

Part:BBa_K2984079:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


Carbonicanhydrase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 241
    Illegal XhoI site found at 427
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 262
    Illegal PstI site found at 609
    Illegal PstI site found at 982
    Illegal NgoMIV site found at 364
    Illegal NgoMIV site found at 1251
    Illegal AgeI site found at 1192
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was used to make our composite parts compatible for the iGEM registry. This part is not MoClo compatible because it does not have the MoClo overhangs!


Source

Carbonicanhydrase

References